Simple Sickling Test Procedure
A Step-by-Step Laboratory Guide
The sickling test is a simple screening method used in hematology laboratories to detect the presence of HbS (sickle hemoglobin) associated with Sickle Cell Disease. The test uses a reducing agent (usually sodium metabisulphite) to remove oxygen from red blood cells, causing cells containing HbS to become sickle-shaped.
Course Content
Simple Sickling Test Procedure
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Materials and Reagents
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Step 1: Prepare the Slide
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Add Sickling Reagent
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Cover the Preparation
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Seal the Edges
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Incubation
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Microscopic Examination
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Interpretation of Results
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Principle of the Sickling Test
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Limitations of the Test
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Confirmatory Tests
