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Course Content
Introduction to ELISA Testing
Learning Objectives By the end of this module, participants should be able to: Define ELISA and its purpose. Explain the principles behind antigen-antibody reactions. Understand the history and development of ELISA. Identify common applications of ELISA in clinical and research settings.
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1.1 Introduction to Immunology
1.2 Antigens and Antibodies
1.3 Principle of ELISA Technology
1.4 Components of an ELISA Assay
1.5 Antigen-Antibody Interaction
1.6 Clinical Applications of ELISA
1.7 Advantages of ELISA
1.8 Limitations of ELISA
1.9 Practical Laboratory Exercise
Module 1 Summary
Understanding ELISA Testing (Enzyme-Linked Immunosorbent Assay)

What is Immunology?

Immunology is the branch of biomedical science that studies the immune system and its response to foreign substances known as antigens. The immune system protects the body against infections, toxins, and abnormal cells through a complex network of cells, tissues, and molecules.

Components of the Immune System

  • White Blood Cells (Leukocytes)
  • Lymphocytes (B-cells and T-cells)
  • Antibodies (Immunoglobulins)
  • Complement System
  • Lymph Nodes
  • Spleen
  • Bone Marrow

Importance of Immunology in Laboratory Medicine

Understanding immunology is essential because ELISA is based on detecting specific immune reactions between antigens and antibodies.

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