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Course Content
Introduction to ELISA Testing
Learning Objectives By the end of this module, participants should be able to: Define ELISA and its purpose. Explain the principles behind antigen-antibody reactions. Understand the history and development of ELISA. Identify common applications of ELISA in clinical and research settings.
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1.1 Introduction to Immunology
1.2 Antigens and Antibodies
1.3 Principle of ELISA Technology
1.4 Components of an ELISA Assay
1.5 Antigen-Antibody Interaction
1.6 Clinical Applications of ELISA
1.7 Advantages of ELISA
1.8 Limitations of ELISA
1.9 Practical Laboratory Exercise
Module 1 Summary
Understanding ELISA Testing (Enzyme-Linked Immunosorbent Assay)

False Positive Results

May occur due to:

  • Cross-reactivity
  • Contamination
  • Poor washing

False Negative Results

May occur due to:

  • Early infection
  • Low analyte concentration
  • Improper specimen handling

Requires Skilled Personnel

Accurate pipetting and interpretation are critical.

Cannot Always Confirm Active Disease

For example, antibodies may persist after recovery.

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