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Course Content
Introduction to ELISA Testing
Learning Objectives By the end of this module, participants should be able to: Define ELISA and its purpose. Explain the principles behind antigen-antibody reactions. Understand the history and development of ELISA. Identify common applications of ELISA in clinical and research settings.
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1.1 Introduction to Immunology
1.2 Antigens and Antibodies
1.3 Principle of ELISA Technology
1.4 Components of an ELISA Assay
1.5 Antigen-Antibody Interaction
1.6 Clinical Applications of ELISA
1.7 Advantages of ELISA
1.8 Limitations of ELISA
1.9 Practical Laboratory Exercise
Module 1 Summary
Understanding ELISA Testing (Enzyme-Linked Immunosorbent Assay)

Infectious Disease Diagnosis

HIV Testing

Detection of:

  • HIV antibodies
  • HIV p24 antigen

Hepatitis B Testing

Detection of:

  • HBsAg
  • Anti-HBs
  • HBeAg

Hepatitis C Testing

Detection of anti-HCV antibodies.

Hormone Testing

ELISA is used to measure:

  • β-hCG (Pregnancy)
  • TSH
  • FSH
  • LH
  • Prolactin
  • Cortisol

Tumor Marker Detection

Examples:

  • PSA (Prostate-Specific Antigen)
  • AFP (Alpha-Fetoprotein)
  • CA-125
  • CEA

Autoimmune Diseases

Detection of:

  • ANA
  • Anti-dsDNA
  • Rheumatoid Factor

Allergy Testing

Detection of:

  • Total IgE
  • Specific IgE antibodies
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