Course Content
Introduction to ELISA Testing
Learning Objectives By the end of this module, participants should be able to: Define ELISA and its purpose. Explain the principles behind antigen-antibody reactions. Understand the history and development of ELISA. Identify common applications of ELISA in clinical and research settings.
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Understanding ELISA Testing (Enzyme-Linked Immunosorbent Assay)

Antigens

An antigen is any substance capable of stimulating an immune response and binding specifically to an antibody.

Examples:

  • Viruses (HIV, HBV, HCV)
  • Bacteria
  • Parasites
  • Hormones
  • Tumor markers
  • Drugs

Types of Antigens

  1. Exogenous Antigens
    • Enter from outside the body.
    • Example: Hepatitis B Surface Antigen (HBsAg)
  2. Endogenous Antigens
    • Produced within cells.
    • Example: Viral proteins are synthesized in infected cells.
  3. Autoantigens
    • Normal body proteins that trigger autoimmune reactions.

Antibodies (Immunoglobulins)

Antibodies are proteins produced by B lymphocytes in response to antigen exposure.

Structure of an Antibody

An antibody consists of:

  • Two heavy chains
  • Two light chains
  • Variable region (antigen binding site)
  • Constant region

Functions of Antibodies

  • Neutralize pathogens
  • Activate complement
  • Promote phagocytosis
  • Prevent the spread of infection

Classes of Immunoglobulins

IgG

  • Most abundant antibody.
  • Indicates past infection or immunity.
  • Crosses the placenta.

IgM

  • The first antibody is produced after infection.
  • Indicates recent or acute infection.

IgA

  • Found in saliva, tears, breast milk, and mucosal surfaces.

IgE

  • Associated with allergies and parasitic infections.

IgD

  • Functions mainly as a receptor on B-cells.