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Course Content
Introduction to ELISA Testing
Learning Objectives By the end of this module, participants should be able to: Define ELISA and its purpose. Explain the principles behind antigen-antibody reactions. Understand the history and development of ELISA. Identify common applications of ELISA in clinical and research settings.
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1.1 Introduction to Immunology
1.2 Antigens and Antibodies
1.3 Principle of ELISA Technology
1.4 Components of an ELISA Assay
1.5 Antigen-Antibody Interaction
1.6 Clinical Applications of ELISA
1.7 Advantages of ELISA
1.8 Limitations of ELISA
1.9 Practical Laboratory Exercise
Module 1 Summary
Understanding ELISA Testing (Enzyme-Linked Immunosorbent Assay)

Microplate

Typically, a 96-well polystyrene plate is designed to bind proteins.

Antigen

The target molecule is being detected.

Antibody

A specific protein is used to recognize the target.

Enzyme Conjugate

Common enzymes include:

  • Horseradish Peroxidase (HRP)
  • Alkaline Phosphatase (ALP)

Substrate

A chemical that reacts with the enzyme to produce color.

Examples:

  • TMB (Tetramethylbenzidine)
  • OPD (Ortho-Phenylenediamine)

Stop Solution

Stops the enzyme reaction and stabilizes color development.

Commonly:

  • Sulfuric Acid (H₂SO₄)
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